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Color Compensation of Multicolor FISH Images
H. Choi, K.R. Castleman and A.C. Bovik
IEEE Transactions on Medical Imaging
Abstract
Multicolor fluorescence in situ hybridization
(M-FISH) techniques provide color karyotyping that allows
simultaneous analysis of numerical and structural abnormalities
of whole human chromosomes. Chromosomes are stained combinatorially
in M-FISH. By analyzing the intensity combinations
of each pixel, all chromosome pixels in an image are classified.
Due to the overlap of excitation and emission spectra and the
broad sensitivity of image sensors, the obtained images contain
crosstalk between the color channels. The crosstalk complicates
both visual and automatic image analysis and may eventually
affect the classification accuracy in M-FISH. The removal of
crosstalk is possible by finding the color compensation matrix,
which quantifies the color spillover between channels. However,
there exists no simple method of finding the color compensation
matrix from multichannel fluorescence images whose specimens
are combinatorially hybridized. In this paper, we present a method
of calculating the color compensation matrix for multichannel
fluorescence images whose specimens are combinatorially stained.
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